Tag

Part:BBa_K549019

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


10x His-tag

This tag is an improvement to the 6x His-tag (K157011). It is designed in order to facilitate the purification of proteins fused to this tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 6
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal NgoMIV site found at 23
    Illegal AgeI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None